Coding

Part:BBa_K4632004:Design

Designed by: Haolong Lai   Group: iGEM23_SCAU-China   (2023-10-09)


Serine protease inhibitor(TIA) fused with ZnuA signal peptide


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 532
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 532
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 532
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 532
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 532
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We found that the TIA protease inhibitor gene, with or without the ZnuA signal peptide, could not be expressed in Escherichia coli. We concluded that E. coli might not be suitable for the expression of the TIA protease inhibitor gene.

Source

TIA source: Huijiao Lin, Richou Han, Xue Qiu, Xun Hongyan. Transgene enterobacter cloacae CEI-a and its construction method and use: CN101144067B[P] 2011-08-10. We have optimized the codons and ordered the plasmid pET28b-ZnuA-TIA, which contains a synthesized ZnuA-TIA fragment, from GUANGZHOU IGE BIOTECHNOLOGY LTD.

References

Huijiao Lin, Richou Han, Xue Qiu, Xun Hongyan. Transgene enterobacter cloacae CEI-a and its construction method and use: CN101144067B[P] 2011-08-10.